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Bio Analysis
biotechnological area should be covered with the state-of-the-art instrumentations and cutting edge technologies with affordable cost.
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Welcome to Relentless Systems

Bio Analysis

  • The following biotechnological area should be covered with the state-of-the-art instrumentations and cutting edge technologies with affordable cost.
  • Bacteria, Fungi, Actinomycetes, Algae Molecular Identification

  • 16S rRNA gene PCR amplification.
  • Internal Transcribed Spacers (ITS) region 1 and 4 gene PCR amplifications.
  • 18S rRNA gene PCR amplification & sequencing for fungi and algae.

DNA Sequencing

  • 16S rRNA gene.
  • Internal Transcribed Spacers (ITS) gene.
  • 18S rRNA gene.
  • Any specific gene (e.g. Resistant gene, enzyme gene, metabolites gene, etc.)

DNA Barcoding for Plants and Animals

  • ITS gene - Plants.
  • Plastid trnH-psbA Intergenic spacer gene - Plants.
  • Chloroplast gene rbcL and matK gene - Plants.
  • Mitochondrial cytochrome C oxidase sub unit 1 gene - Animals.
  • Biocode LIMS and GenBank submission.

Molecular techniques and Recombinant Technology

  • Polymerase Chain Reaction.
  • Cloning, Vector Construction.
  • Restriction Fragment Length Polymorphisms (RFLP).
  • Amplified Fragment Length Polymorphisms (AFLP).
  • Amplified Ribosomal DNA Restriction Analysis (ARDRA).
  • Random Amplification of Polymorphic DNA (RAPD).
  • cDNA Library Construction.
  • Competent Cell Preparation.
  • Total DNA/RNA Isolation.
  • Gel Electrophoresis (SDS-PAGE, Native PAGE, Agarose).
  • Enzyme-Linked Immunosorbent Assay (ELISA).
  • Southern and Western Blot analysis.
  • cDNA Library Construction.

Biotechnological Database Analyses

  • BLAST Analysis.
  • Pubmed Search.
  • GenBank Submission.
  • RDP Analysis.
  • Swissprot Analysis.
  • Mascot Programe.
  • MEGA, Treecon, Clustal W and Chromas Analysis for Phylogeny Tree Constructions.

In vitro Bioassays

  • Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoan, Antialgal, Anticandidal).
  • Anticancer activity (viz., Cell lines, Cytotoxicity, MTT assay, DNA fragmentation, Apoptotic Protein expression).
  • Antioxidant activity (DPPH scavenging, ABTS scavenging, FRAP, TRAP, Reducing power).
  • Antiinflammatory, Antilipidimic, Antidiabetic activity.
  • Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics, Enzyme applications).

Phytochemical Analysis

  • Total Phenols estimation.
  • Alkaloid content estimation.
  • Flavanoid content estimation.
  • Terpenoid content estimation.
  • Saponin content estimation.
  • Vitamins and minerals content.
  • Nutritional analysis.
  • Total Carbohydrate, Protein and lipid content.
  • Total fiber and ash content.
  • Melanin content.

Purification of Active molecules and Enzymes

  • Bioactive compound purification from Bacteria, Actinomycetes, Fungi, Algae, Medicinal Plants, Animals sources.
  • Extracellular or Intracellular metabolites purification.
  • Silica Gel Column Chromatography (Low/High resolution) for Secondary metabolites.
  • Sephadex LH20 Column Chromatography for Secondary metabolites.
  • Ion Exchange Column Chromatography form Protein/Carbohydrates
  • Size Exclusion Column Chromatography (Gel Permeation Column Chromatography).
  • Affinity Column Chromatography.
  • Flash Chromatography.
  • Thin Layer Chromatography (TLC).
  • Gas Chromatography (GC).
  • Preparative High Performance Liquid Chromatography.

Spectral Analyses and Secondary Metabolites Structure Elucidation

  • UV-Vis Spectrometry (UV-Vis spectrum).
  • Infra Red spectrum (FT-IR, KBr pellet, UV-Vis-NIR).
  • NMR spectrum (HNMR, CNMR, 2D NMR).
  • Mass Spectrum (GC-MS, ISI-MS, Maldi-Tof MS, LC-MS, LC_MS/MS).
  • High Performance Liquid Chromatography (HPLC).
  • High Performance Thin Layer Chromatography (HPTLC).
  • X-ray Diffraction (Single crystal XRD / Powder XRD).
  • Atomic absorption Spectorscopy (AAS) and CHNSO Analysis.
  • Inductively coupled plasma mass spectrometry (ICP-MS) .
  • Electron Microscopy (SEM, TEM, AFM, Phase Contrast) Analysis.
  • Molecular structure elucidation.

Microbiological Analyses

  • PCR Identification of pathogens.
  • Quality assurance for foods, agriculture inputs and environmental samples.
  • Microbial load analysis.
  • Bioaugmentation analysis.

Other analysis

  • Heavy metal analysis.
  • Nutritional analysis.
  • Metabolite analysis.
  • Compound/drug quantification
  • Adulterations identifications.
  • Soil Analysis.
  • Water quality analysis.