home

results driven

iT Solutions & Services
Our professional services help you address the ever evolving business and technological challenges.

web design & development

Visually Compelling & Functional
Online marketing
Relentless Systems enhance your brand, your website traffic and your bottom line online.

web hosting

Relentless Systems offer the web's best hosting plans for every site.
domain name registration
Secure your online identity and register your domain now.

video production
Make a greater impact on your clients through interactive Video Production.

Bio Analysis
biotechnological area should be covered with the state-of-the-art instrumentations and cutting edge technologies with affordable cost.
mobile applications
Stay connected to your customers on the go with a MajorMedia custom mobile app.
bootstrap slider

Welcome to Relentless Systems

Bio Analysis

  • The following biotechnological area should be covered with the state-of-the-art instrumentations and cutting edge technologies with affordable cost.
  • Bacteria, Fungi, Actinomycetes, Algae Molecular Identification

  • 16S rRNA gene PCR amplification.
  • Internal Transcribed Spacers (ITS) region 1 and 4 gene PCR amplifications.
  • 18S rRNA gene PCR amplification & sequencing for fungi and algae.

DNA Sequencing

  • 16S rRNA gene.
  • Internal Transcribed Spacers (ITS) gene.
  • 18S rRNA gene.
  • Any specific gene (e.g. Resistant gene, enzyme gene, metabolites gene, etc.)

DNA Barcoding for Plants and Animals

  • ITS gene - Plants.
  • Plastid trnH-psbA Intergenic spacer gene - Plants.
  • Chloroplast gene rbcL and matK gene - Plants.
  • Mitochondrial cytochrome C oxidase sub unit 1 gene - Animals.
  • Biocode LIMS and GenBank submission.

Molecular techniques and Recombinant Technology

  • Polymerase Chain Reaction.
  • Cloning, Vector Construction.
  • Restriction Fragment Length Polymorphisms (RFLP).
  • Amplified Fragment Length Polymorphisms (AFLP).
  • Amplified Ribosomal DNA Restriction Analysis (ARDRA).
  • Random Amplification of Polymorphic DNA (RAPD).
  • cDNA Library Construction.
  • Competent Cell Preparation.
  • Total DNA/RNA Isolation.
  • Gel Electrophoresis (SDS-PAGE, Native PAGE, Agarose).
  • Enzyme-Linked Immunosorbent Assay (ELISA).
  • Southern and Western Blot analysis.
  • cDNA Library Construction.

Biotechnological Database Analyses

  • BLAST Analysis.
  • Pubmed Search.
  • GenBank Submission.
  • RDP Analysis.
  • Swissprot Analysis.
  • Mascot Programe.
  • MEGA, Treecon, Clustal W and Chromas Analysis for Phylogeny Tree Constructions.

In vitro Bioassays

  • Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoan, Antialgal, Anticandidal).
  • Anticancer activity (viz., Cell lines, Cytotoxicity, MTT assay, DNA fragmentation, Apoptotic Protein expression).
  • Antioxidant activity (DPPH scavenging, ABTS scavenging, FRAP, TRAP, Reducing power).
  • Antiinflammatory, Antilipidimic, Antidiabetic activity.
  • Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics, Enzyme applications).

Phytochemical Analysis

  • Total Phenols estimation.
  • Alkaloid content estimation.
  • Flavanoid content estimation.
  • Terpenoid content estimation.
  • Saponin content estimation.
  • Vitamins and minerals content.
  • Nutritional analysis.
  • Total Carbohydrate, Protein and lipid content.
  • Total fiber and ash content.
  • Melanin content.

Purification of Active molecules and Enzymes

  • Bioactive compound purification from Bacteria, Actinomycetes, Fungi, Algae, Medicinal Plants, Animals sources.
  • Extracellular or Intracellular metabolites purification.
  • Silica Gel Column Chromatography (Low/High resolution) for Secondary metabolites.
  • Sephadex LH20 Column Chromatography for Secondary metabolites.
  • Ion Exchange Column Chromatography form Protein/Carbohydrates
  • Size Exclusion Column Chromatography (Gel Permeation Column Chromatography).
  • Affinity Column Chromatography.
  • Flash Chromatography.
  • Thin Layer Chromatography (TLC).
  • Gas Chromatography (GC).
  • Preparative High Performance Liquid Chromatography.

Spectral Analyses and Secondary Metabolites Structure Elucidation

  • UV-Vis Spectrometry (UV-Vis spectrum).
  • Infra Red spectrum (FT-IR, KBr pellet, UV-Vis-NIR).
  • NMR spectrum (HNMR, CNMR, 2D NMR).
  • Mass Spectrum (GC-MS, ISI-MS, Maldi-Tof MS, LC-MS, LC_MS/MS).
  • High Performance Liquid Chromatography (HPLC).
  • High Performance Thin Layer Chromatography (HPTLC).
  • X-ray Diffraction (Single crystal XRD / Powder XRD).
  • Atomic absorption Spectorscopy (AAS) and CHNSO Analysis.
  • Inductively coupled plasma mass spectrometry (ICP-MS) .
  • Electron Microscopy (SEM, TEM, AFM, Phase Contrast) Analysis.
  • Molecular structure elucidation.

Microbiological Analyses

  • PCR Identification of pathogens.
  • Quality assurance for foods, agriculture inputs and environmental samples.
  • Microbial load analysis.
  • Bioaugmentation analysis.

Other analysis

  • Heavy metal analysis.
  • Nutritional analysis.
  • Metabolite analysis.
  • Compound/drug quantification
  • Adulterations identifications.
  • Soil Analysis.
  • Water quality analysis.
top